mouse cd86 pe 65068  (Proteintech)

Journal: Redox Biology

Article Title: Redox-modulating macrophage biohybrid nanoplatform for targeted RIPK1-PANoptosome suppression in ischemic stroke

doi: 10.1016/j.redox.2025.103997

Figure Lengend Snippet: In vitro evaluation of microglia regulation of MA@ULips. (a) Scheme of microglia phenotype transition induced by MA@ULips. (b) Flow cytometry analysis of CD86 and CD206 in BV2 cells after diverse treatments. Representative immunofluorescence staining images of (c) CD86 and (d) CD206 in BV2 cells after various treatments. The level of (e) Arg-1, (f) IL-10, (g) TNF-α, and (h) IL-6 in the BV2 cells after different treatments (n = 3). Data are presented as mean ± SD. Statistical significance was calculated by one-way ANOVA with Tukey's multiple comparisons test. ∗ p < 0.05, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Anti-caspase 1 (31020-1-AP), anti-RIPK1 (17519-1-AP) and PE- anti -mouse CD86 + (PE-65068) were obtained from Proteintech Group.

Techniques: In Vitro, Flow Cytometry, Immunofluorescence, Staining

Journal: Redox Biology

Article Title: Redox-modulating macrophage biohybrid nanoplatform for targeted RIPK1-PANoptosome suppression in ischemic stroke

doi: 10.1016/j.redox.2025.103997

Figure Lengend Snippet: MA@ULips enhancing neurological functional recovery and modulating ischemic inflammatory microenvironment. (a) Experimental design involved multiple functional tests to evaluate the neurological outcomes of MA@ULips. (b) Forelimb asymmetry rate assessed through the cylinder test (n = 8). (c) Motion paths in the open field test. (d) Quantitative analysis of movement distance (n = 7). (e) Representative images of sham-operated mice and MCAO/R mice after different treatments. (f) Neurological deficit evaluation by the mNSS (n = 6). (g) Immunohistochemical staining for GFAP on brain tissue from the ischemic cortex area after different treatments. (h) Immunofluorescent staining of brain tissue for Iba-1 and CD86 after different treatments. (i–j) The levels of (i) IL-10 and (j) IL-6 in serum of ischemic mice after different treatments (n = 3). (k–l) The levels of (k) IL-6 and (l) IL-10 in brain tissues of ischemic mice after different treatments (n = 3). Data are presented as mean ± SD. Statistical significance was calculated by one-way ANOVA with Tukey's multiple comparisons test. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Anti-caspase 1 (31020-1-AP), anti-RIPK1 (17519-1-AP) and PE- anti -mouse CD86 + (PE-65068) were obtained from Proteintech Group.

Techniques: Functional Assay, Immunohistochemical staining, Staining

Journal: Materials Today Bio

Article Title: Self-assembly of paclitaxel derivative and fructose as a potent inducer of immunogenic cell death to enhance cancer immunotherapy

doi: 10.1016/j.mtbio.2025.101793

Figure Lengend Snippet: Immunological analysis in vitro (n = 3). (A) Flow cytometry analysis and quantification of CD80 and CD86 double-positive DC cells. (B) Flow cytometry analysis of M1 macrophages (CD86 + CD206 - ) and M2 macrophages (CD86 - CD206 + ) in BMDM cells following various treatments and the corresponding ratio of M1/M2. Data are presented as mean ± SD. Statistical significance is indicated as & p < 0.05, && p < 0.01 vs PTX injections; ∗p < 0.05, ∗∗∗p < 0.001 vs PTX-PBA-Fru NPs + R848-Lipo (one-way ANOVA followed by Tukey's multiple comparisons test).

Article Snippet: They were stained with the Zombie VioletTM Fixable Viability Kit (BV421, BioLegend) at room temperature in the dark for 15 min. After washing with CSB and centrifugation at 3500 rpm for 5 min, the cells were then resuspended in CSB and incubated with purified anti-mouse CD16/32 antibody (Elabscience) for 15 min. Next, the cells were stained with APC anti-mouse CD45 (Proteintech), F4/80 monoclonal antibody (PerCP-Cyanine5.5, eBioscience), and PE anti-mouse CD86 (Proteintech) for 30 min. After another wash and centrifugation, the cells were resuspended in CSB and fixed for 10 min with 0.5 mL of pre-cooled fixation buffer (Shanghai Yuanye Bio-Technology Co., Ltd.) at room temperature.

Techniques: In Vitro, Flow Cytometry